Journal: Frontiers in Bioengineering and Biotechnology
Article Title: High-throughput 3D phenotypic screening identifies repurposed MEK inhibitors as drivers of chondrogenesis for cartilage regeneration
doi: 10.3389/fbioe.2026.1748443
Figure Lengend Snippet: (a) Fluorescent images of samples treated with Trametinib, stained with DAPI (blue), SOX9 (green), and Collagen II (gray) at selected concentration levels (10, 30, and 100 nM). Scale bars = 50 µm. (b) Quantification of fluorescent Collagen II signals from image analysis. Data are expressed as mean ± SD, with statistical significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns: not significant.
Article Snippet: Following fixation, samples were permeabilized and blocked with a solution of 2% bovine serum albumin (BSA) and 0.1% Triton-X in PBS for 2 h. Primary antibody incubation was conducted overnight at 4 °C using a goat anti-human SOX9 antibody (10 μg/mL, R&D Systems) and a rabbit anti-human Collagen II antibody (1:500 dilution, Sigma Aldrich).
Techniques: Staining, Concentration Assay